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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in advertising and marketing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two further splice variants were described but comprehensive nucleotide sequence information and facts is just not but out there.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually is made up of the epitope recognized via the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the very best outcomes are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope within the focus on protein. By evaluating the staining from the blocked antibody versus the antibody by itself, you can see which staining is certain.
Concentrate on Info Cyclin E belongs towards the hugely conserved cyclin relatives, whose customers are characterised by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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